The Natural History Database and Biosample Repository has been instrumental in advancing my LAM research. The extensive resources within the database have been valuable for my investigations, and the accessibility to rare biosamples within the repository has significantly enhanced my work. The constant support provided by the TSC Alliance has been valuable in advancing my research.”
Amina Jouida, PhD, University College Dublin (BSR Seed Grant Awardee 2022)
The TSC Biosample Repository houses human biological materials such as blood, DNA, and tissues linked to detailed clinical data in the TSC Natural History Database. High-quality biosamples and their associated clinical data will enable researchers to discover biomarkers, establish human cell lines or tissue arrays for drug testing, and search for clues to understand why TSC is so different from person to person.
Types of samples available include:
- DNA isolated from white blood cells and buccal cells
- White blood cell pellets
- Remnant tissue from surgeries (frozen or fixed, paraffin-embedded), including brain, kidney, liver, etc.
How to request samples
Please download the Application for Biosample Access and follow the instructions on pages 1-2. Prior to applying, we encourage you to discuss your project’s goals and biosample needs by emailing firstname.lastname@example.org.
All biosample requests will be reviewed by the Natural History Database and Biosample Repository Steering Committee.
HEK293T cell lines
The TSC Biosample Repository also provides researchers in the Americas access to the TSC1- and TSC2-knockout HEK293T cell lines from the Nellist laboratory at Erasmus MC:
- Cell line 1C2 (TSC1-/-)
- Cell line 3H9 (TSC2-/-)
- Cell line 3H9-1B1 (TSC1-/-/TSC2-/-)
- HEK 293T (parental cell line)
The Van Andel Research Institute in Grand Rapids, MI processes, stores, and delivers TSC Biosample Repository samples on behalf of the TSC Alliance. Download the HEK Cell Line Request Form. Replacement cell lines have a flat rate cost of $200 to cover shipping and staff time.
The TSC Alliance is eager to partner with sponsors of clinical trials or clinical research studies to collect biosamples centrally at the TSC Biosample Repository. Such biosamples will remain under the control of the study’s biosample use committee until the conclusion of the project, at which time the samples will become part of the openly available TSC Biosample Repository. This provides a win-win opportunity to ensure the long-term availability of valuable samples. This process is being used with the Developmental Synaptopathies Consortium and the TSC-STEPS clinical trial.
Information for individuals with TSC and their families
If you are an individual with TSC or a family member of someone with TSC, please see here for information about donating samples to the TSC Biosample Repository.
The TSC Natural History Database and Biosample Repository are governed and wholly funded by the TSC Alliance thanks to generous support from Lorne Waxlax, William Watts, Dr. Michael and Janie Frost, Janice and Julian Gangolli Family Fund, Jim and the late Andrea Maginn, and many additional donors through the Unlock the Cure campaign.